Efficacy of combined albendazol and praziquntel and their loaded solid lipid nanoparticles components in chemoprophylaxis of experimental hydatidosis

Objective To evaluate the efficacy of combined ABZ and PZQ and their solid lipid nanoparticles in chemoprophylaxis of cystic echinococcosis (CE). Methods ABZ and PZQ loaded solid lipid nanoparticles (SLNs) were prepared by high shear homogenization and microemulsion congealing techniques with some minor modification. Nanoparticles average size, polydispersity index (PDI), and particle size distribution were determined by scanning electron microscopy (SEM) and photon correlation spectroscopy. Forty females BALB/c were experimentally infected by protoscoleces (PSC) and randomly divided into four equal groups of 10 mice. After the end of the 3 months treatment period and 2 months rest, mice were sacrificed and the peritoneal cavity was opened for removal, counting, measuring, and histological analysis of hydatid cyst. Results The results indicated that ABZ and PZQ chemoprophylaxis treatment reduced the wet weight and size of developed cysts 77.3% and 79%, respectively. The corresponding result for the ABZ and PZQ loaded SLNs was 83% and 85%, respectively. Conclusions This study for the first time demonstrated that ABZ and PZQ loaded SLNs is superior to free ABZ and PZQ for the chemoprophylaxis of CE in mice.

[Detection of intestinal parasite agents on raw consumed vegetables in Ahvaz]

The prevalence of parasitic disease is one of the major human hygienic problems in more countries especially in developing country such as Iran and it reached as high as 90% in some area of the world. The presence of parasitic disease has also observed in various rates in Iran .The important ways of parasite infection is oral route and raw consumption of vegetable. This research is a descriptive epidemiological study for evaluating vegetable contamination with parasitic agents. In this study, 40 samples of vegetable are collected from farms in four different geographical area around Ahvaz. The sedimentation method was used with anionic detergent as described by FAO method. In this study, nematode eggs 60%, larva of nematodes in the third stage 40%, intestinal protozoan 10%, have been seen. The abandonment of the use of human compost in agriculture Prevention of using of compost human in agriculture, hygienic washing of vegetable in endemic area and teaching principles of public health to people can control parasitic diseases and promote hygiene level of public health